Adoption of Innovative technology: Seed to fattening of mud crab (Scylla olivacea) and health management in Bangladesh condition শিরনামে চলমান প্রকল্পের অগ্রগতি

Project ID: SP-029

Project Fund: National Agricultural Technology Project (NATP): Phase-2 (BARC Component).

            Total budget: BDT26254768.00

            Budget for KU component:  BDT8025000.00

Project period: May, 2018…………  End: April 2021

Implementing institution/organization:

Coordination component: Shrimp Research Centre, BFRI, Bagerhat,  Bangladesh.

Component-1: Nutrition Unit; BARC; Dhaka-1215.

Component-2: Bangladesh Fisheries Research Institute (BFRI),

Brackish water Station,  Paikgacha, Khulna- 9280.

            Component- 3: Fisheries and Marine Resource Technology Discipline, Khulna

                                              University, Khulna-9208.



Dr. Khan KAmal Uddin Ahmed

                                                Chief Scientific Officer

Shrimp Research Centre, BFRI, Bagerhat,  Bangladesh


Component- 3 Personnel:

                  PI:        Abul Farah Md. Hasanuzzaman, PhD

Associate Professor

Fisheries and Marine Resource Technology Discipline.


Co-PI: Dr. Ghausiatur Reza Banu

Professor, Fisheries and Marine Resource Technology Discipline.


PhD Student: Md. Rashedul Islam


            Lab Attendant: Md. Bappi Forazi



Goal: Development and promotion of improved technology for sustainable production of mud crab for qualitative and quantitative supply of seed and adult exportable mud crab.


Objective of the project:


General objective

Development and establish a framework of mud crab aquaculture for sustainable production through conducting research on each critical stages of life cycle in accounting the pathogenic (microbial) threats on respective stages.


Specific objective

(KU Component)

i) To quantify bacterial load and type/infection level in Mud crab hatchery, farms and in wild mud crab, S. olivacea.

ii)          To record disease incidence in mud crab (S. olivacea) population in the south-west coastal region of Bangladesh

iii)        To determine the association of other driving factors (i.e. feed, soil and water quality) with infection/diseases occurrence in mud crab, S. olivacea.


Component- 3 Approach(s):


Samples of crabs, water and soil will be collected from mud crab hatchery and ponds/pens and all the trials conducted by BFRI, BS. Mud crabs are also being collected from the waters in and around the Sundarbans areas. Samples are being preserved following standard approaches (e.g. APHA, BAM). Water parameters such as temperature, dissolved oxygen, pH, salinity and soil parameters (e.g. pH, total carbon, organic carbon, total nitrogen) will be checked using measuring instruments, kit, and standard methods (e.g. APHA, AOAC). Bacteria load and isolation will be determined; all steps for isolation and enumeration will be performed according to the standard guidelines (BAM/EPA/AOAC). Several biochemical tests will be carried out to confirm the isolates. In addition, PCR method (end-point approach) will be applied to confirm the presence of pathogenic bacteria such as Vibrio spp., Pseudomonas spp., Aeromonas spp. in mud crabs. For preliminary diagnosis of diseased and/or infected crabs, gross observations (i.e. external signs, discoloured patches, soft and black exoskeleton, orange or brown spot on shell) will be performed, and compared with the healthy crabs collected from the same ponds/pens. For details, histopathology will be performed following standard procedures.

Expected outputs/results of the Project:   

·         Generation of a comprehensive knowledge on mud crab aquaculture.

·         Development of mud crab seed production protocols in hatchery condition.

·         Establishment of nursery protocols for enhanced survival.

·         Detection of bacterial infection in mud crab population of hatchery, farms and wild in and around the south-western Sundarbans region of Bangladesh.

·         Understanding of driving factors associated with diseases occurrence in mud crab population.

·         Capacity build-up of crab fatteners and project-investigators toward sustainable health management for mud crab fattening in Bangladesh.


Target beneficiaries: Crab farmers, depot owners and exporters, brackish water hatchery managers, nursery owners, academics, researcher and students involved in fisheries and aquaculture research.